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Metabolic reprogramming in cancer: dysregulation of glucose, lipid, and amino acid pathways and therapeutic opportunities | Molecular Biomedicine | Springer Nature Link
A data-independent acquisition MS method was used for proteome and phosphoproteome analysis in which one full scan (300–1,650 m/z , R = 60,000 at 200 m/z ) at a target of 3 × 10 6 ions was first performed, followed by 32 windows with a resolution of 30,000 where precursor ions were fragmented with higher energy collisional dissociation (stepped collision energy 25%, 27.5%, 30%) and analyzed with an AGC target of 3 × 10 6 ions and a maximum injection time at 54 ms in profile mode using positive polarity
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The molecule was discovered more than 100 years ago, and scientists have been exploring the longevity potential for over two decades